This is cutadapt 1.18 with Python 3.6.8 Command line parameters: -q 10 -a AGATCGGAAGAGC -A AGATCGGAAGAGC -o SRR7825135_1.trim.fastq -p SRR7825135_2.trim.fastq SRR7825135_1.fastq SRR7825135_2.fastq Processing reads on 1 core in paired-end mode ... Finished in 528.68 s (48 us/read; 1.25 M reads/minute). === Summary === Total read pairs processed: 11,024,477 Read 1 with adapter: 260,264 (2.4%) Read 2 with adapter: 246,760 (2.2%) Pairs written (passing filters): 11,024,477 (100.0%) Total basepairs processed: 2,204,895,400 bp Read 1: 1,102,447,700 bp Read 2: 1,102,447,700 bp Quality-trimmed: 81,165,611 bp (3.7%) Read 1: 23,489,189 bp Read 2: 57,676,422 bp Total written (filtered): 2,121,229,741 bp (96.2%) Read 1: 1,077,668,280 bp Read 2: 1,043,561,461 bp === First read: Adapter 1 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 260264 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 29.9% C: 26.2% G: 17.3% T: 26.4% none/other: 0.3% Overview of removed sequences length count expect max.err error counts 3 165230 172257.5 0 165230 4 59975 43064.4 0 59975 5 13024 10766.1 0 13024 6 2944 2691.5 0 2944 7 1291 672.9 0 1291 8 833 168.2 0 833 9 1119 42.1 0 957 162 10 1067 10.5 1 855 212 11 1020 2.6 1 880 140 12 805 0.7 1 769 36 13 597 0.2 1 578 19 14 541 0.2 1 526 15 15 478 0.2 1 459 19 16 464 0.2 1 446 18 17 521 0.2 1 503 18 18 474 0.2 1 452 22 19 525 0.2 1 508 17 20 674 0.2 1 659 15 21 656 0.2 1 639 17 22 579 0.2 1 551 28 23 393 0.2 1 381 12 24 346 0.2 1 328 18 25 291 0.2 1 280 11 26 270 0.2 1 258 12 27 244 0.2 1 233 11 28 271 0.2 1 258 13 29 291 0.2 1 284 7 30 346 0.2 1 337 9 31 352 0.2 1 335 17 32 279 0.2 1 267 12 33 190 0.2 1 175 15 34 177 0.2 1 169 8 35 175 0.2 1 166 9 36 159 0.2 1 148 11 37 125 0.2 1 115 10 38 150 0.2 1 143 7 39 130 0.2 1 122 8 40 164 0.2 1 155 9 41 144 0.2 1 137 7 42 116 0.2 1 105 11 43 87 0.2 1 83 4 44 69 0.2 1 66 3 45 76 0.2 1 72 4 46 102 0.2 1 96 6 47 40 0.2 1 37 3 48 57 0.2 1 52 5 49 77 0.2 1 68 9 50 65 0.2 1 57 8 51 76 0.2 1 66 10 52 72 0.2 1 60 12 53 57 0.2 1 53 4 54 44 0.2 1 35 9 55 38 0.2 1 29 9 56 40 0.2 1 29 11 57 33 0.2 1 28 5 58 46 0.2 1 37 9 59 50 0.2 1 39 11 60 45 0.2 1 32 13 61 48 0.2 1 41 7 62 74 0.2 1 40 34 63 129 0.2 1 87 42 64 116 0.2 1 102 14 65 50 0.2 1 35 15 66 80 0.2 1 45 35 67 87 0.2 1 48 39 68 156 0.2 1 93 63 69 282 0.2 1 137 145 70 338 0.2 1 263 75 71 140 0.2 1 109 31 72 80 0.2 1 63 17 73 30 0.2 1 23 7 74 15 0.2 1 9 6 75 6 0.2 1 3 3 76 10 0.2 1 1 9 77 7 0.2 1 1 6 78 12 0.2 1 1 11 79 10 0.2 1 1 9 80 2 0.2 1 0 2 81 3 0.2 1 0 3 82 5 0.2 1 0 5 83 4 0.2 1 0 4 84 3 0.2 1 0 3 85 7 0.2 1 1 6 86 2 0.2 1 0 2 87 3 0.2 1 0 3 88 5 0.2 1 0 5 89 4 0.2 1 0 4 90 4 0.2 1 0 4 91 4 0.2 1 0 4 92 4 0.2 1 0 4 94 7 0.2 1 0 7 95 4 0.2 1 0 4 96 5 0.2 1 0 5 97 4 0.2 1 0 4 98 4 0.2 1 0 4 99 5 0.2 1 0 5 100 11 0.2 1 1 10 === Second read: Adapter 2 === Sequence: AGATCGGAAGAGC; Type: regular 3'; Length: 13; Trimmed: 246760 times. No. of allowed errors: 0-9 bp: 0; 10-13 bp: 1 Bases preceding removed adapters: A: 30.6% C: 26.2% G: 16.7% T: 26.4% none/other: 0.2% Overview of removed sequences length count expect max.err error counts 3 156893 172257.5 0 156893 4 55564 43064.4 0 55564 5 12630 10766.1 0 12630 6 2926 2691.5 0 2926 7 1336 672.9 0 1336 8 786 168.2 0 786 9 1171 42.1 0 996 175 10 1026 10.5 1 768 258 11 1076 2.6 1 933 143 12 759 0.7 1 716 43 13 744 0.2 1 712 32 14 732 0.2 1 712 20 15 389 0.2 1 374 15 16 578 0.2 1 558 20 17 687 0.2 1 659 28 18 430 0.2 1 412 18 19 594 0.2 1 569 25 20 394 0.2 1 376 18 21 553 0.2 1 527 26 22 483 0.2 1 453 30 23 415 0.2 1 389 26 24 603 0.2 1 573 30 25 240 0.2 1 227 13 26 168 0.2 1 141 27 27 209 0.2 1 198 11 28 294 0.2 1 283 11 29 314 0.2 1 293 21 30 598 0.2 1 578 20 31 204 0.2 1 187 17 32 215 0.2 1 205 10 33 85 0.2 1 79 6 34 118 0.2 1 110 8 35 159 0.2 1 149 10 36 148 0.2 1 142 6 37 113 0.2 1 107 6 38 120 0.2 1 106 14 39 124 0.2 1 111 13 40 159 0.2 1 146 13 41 148 0.2 1 130 18 42 137 0.2 1 129 8 43 106 0.2 1 91 15 44 120 0.2 1 101 19 45 89 0.2 1 79 10 46 63 0.2 1 52 11 47 42 0.2 1 32 10 48 60 0.2 1 50 10 49 52 0.2 1 44 8 50 70 0.2 1 59 11 51 97 0.2 1 90 7 52 24 0.2 1 19 5 53 33 0.2 1 29 4 54 62 0.2 1 51 11 55 42 0.2 1 39 3 56 46 0.2 1 34 12 57 37 0.2 1 29 8 58 68 0.2 1 52 16 59 42 0.2 1 31 11 60 47 0.2 1 37 10 61 49 0.2 1 41 8 62 87 0.2 1 72 15 63 174 0.2 1 145 29 64 207 0.2 1 169 38 65 359 0.2 1 284 75 66 227 0.2 1 175 52 67 44 0.2 1 29 15 68 8 0.2 1 4 4 69 11 0.2 1 5 6 70 5 0.2 1 1 4 71 5 0.2 1 2 3 72 10 0.2 1 0 10 73 4 0.2 1 0 4 74 8 0.2 1 0 8 75 8 0.2 1 0 8 76 5 0.2 1 0 5 77 6 0.2 1 0 6 78 4 0.2 1 0 4 79 8 0.2 1 0 8 80 2 0.2 1 0 2 81 7 0.2 1 1 6 82 5 0.2 1 0 5 83 3 0.2 1 1 2 84 3 0.2 1 0 3 85 6 0.2 1 0 6 86 8 0.2 1 0 8 87 7 0.2 1 0 7 88 3 0.2 1 0 3 89 1 0.2 1 0 1 90 5 0.2 1 0 5 91 3 0.2 1 0 3 92 5 0.2 1 0 5 93 5 0.2 1 0 5 94 5 0.2 1 1 4 95 4 0.2 1 0 4 96 2 0.2 1 0 2 97 4 0.2 1 0 4 98 6 0.2 1 0 6 99 5 0.2 1 0 5 100 20 0.2 1 0 20